DNA Polymerase
From 3D Printables
DNA Polymerase
E. Coli DNA polymerase |
Human DNA polymerase |
Also known as: DNA-dependent DNA polymerase, DNApol
When cells divide, the DNA inside the cell nucleus must be copied so that each new cell has a full set of chromosomes. DNA polymerase attaches to a single strand of DNA and catalyzes the reaction that adds the other half of the double helix. DNA polymerase also performs a "proofreading" function on newly synthesized DNA, ensuring that base pairs are correctly matched.
Different organisms have different DNA polymerases. In the downloads section you will find human DNA polymerase-beta and DNA polymerase from E. Coli.
Contents |
Educational Use
These models can be used together to highlight the differences between enzymes between species. The human and E. Coli forms of this enzyme have noticibly different shapes even though they perform the same basic function.
Each model can also be used separately to demonstrate the importance of proper shape for correct enzymatic action.
Downloads
Human DNA polymerase:
- Polymerase .STL file (zipped, 50MB)
- DNA fragment .STL file (10MB)
E. Coli DNA polymerase:
- Polymerase .STL file (zipped, 88MB)
- DNA fragment .STL file (17MB)
Model Specifications
Both models were rendered in STLGenerator
Settings:
- Resolution: 0.2
- Atom size: 1.2
and printed on a Stratasys SST (STL scale = 1.2)
E. Coli DNA pol - PDB entry 1KLN
Ribbon model of E. Coli DNA polymerase |
E. Coli DNA polymerase in STLGenerator |
Printed model of E. Coli DNA polymerase |
Human DNA pol - PDB entry 1ZQA
Ribbon model of human DNA polymerase |
Human DNA polymerase in STLGenerator |
Printed model of DNA polymerase complexed with 7 base pair fragment of DNA |
Resources
- Protein Data Bank (PDB) Molecule of the Month feature on DNA Polymerase by David Goodsell
- Characterization of the metal ion binding helix-hairpin-helix motifs in human DNA polymerase beta by X-ray structural analysis - Article in which structure of PDB entry 1ZQA was published
Credits
Model created from Protein Data Bank (PDB) entries 1ZQA (human) and 1KLN (E. Coli).
